"Covalent affinity" purification of ubiquitin-activating enzyme.
نویسندگان
چکیده
منابع مشابه
Selective enzyme purification by affinity chromatography.
The purification of proteins by conventional procedures is frequently laborious and incomplete, and the yields are often low. Enzyme isolation based on a highly specific biological property-strong reversible association with specific substrates or inhibitors-has received only limited attention.‘-’ In affinity chromatography, the enzyme to be purified is passed through a column containing a cros...
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Ubiquitin tags diseased proteins and initiates an enzyme conjugation cascade, which has three stages. The first-stage enzyme Uba1 (E1) has evolved only modestly from slime mold to humans, and is > 14 times larger than Ub. Here we use critical point thermodynamic scaling theory to connect Uba1 (E1) evolution from yeast and slime mold to fruit flies and humans to subtle changes in its amino acid ...
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Activation reactions of this type are catalyzed by enzymes from various sources (2-6) and specific activating enzymes may be required for each amino acid (2). The first highly purified, activating enzyme was a tryptophan-activating enzyme from beef pancreas, studied by Davie et al. (7). The isolation and properties of a tyrosine-activating enzyme from hog pancreas (8) are described in this report.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34957-3